Defining new parameters and functions of the force regulatory model

Defining your custom parameter

Click Edit Parameters in the menu bar on your model editor page. Click on the Your Custom Parameter  tab.

Add the following parameters in the Your Custom Parameter form.

// T/T Unit parameters
K_ON = 60.0
K_OFF = 80.0
KP_ON = 60.0
KP_OFF = 30.0

// T/T Unit-MH relations
GAMMA = 40.0
Q = 3.6
MYU = 15.0
K_BASIC = 16.0
K_NP0 = Q * K_BASIC / MYU
K_NP1 = Q * K_BASIC
K_PN0 = K_BASIC * GAMMA**2
K_PN1 = K_BASIC * GAMMA**2
XB_STRONG = 0.4

Then click on the Close button.

Defining a function on the myosin head states

Click Edit Functions in the menu bar. Click on the Add Function button. Click on the tentative function name new_mh_func0.

Input ng in the Function name box and the function return values: 1 for P_XB, 1 for XB_PreR, 1 for XB_PostR and 0 for N_XB. The function ng will be referred in defining the transition rates for the T/T Unit in Defining transitions of the T/T units, and in representing the cooperativity between neighboring myosin heads in Defining transitions with the cooperative behaviors of the myosin heads.

Click on the function name ng to close the function return value form.

Defining functions on the T/T Unit states

Click on the T/T Unit tab to define functions on the T/T unit states.

Click on the Add Function button. Click on the tentative function name new_tt_func0. Input knp in the Function name box and the function return values : K_NP0 for Ca_off and K_NP1 for Ca_on. Here K_NP0 and K_NP1 are the parameters defined in the Your Custom Parameter form.

Click on the function name knp to close the function return value form.

Click again on the Add Function button. Click on the tentative function name new_tt_func1. Input kpn in the Function name box and the function return values: K_PN0 for Ca_off and K_PN1 for Ca_on. Here K_PN0 and K_PN1 are the parameters defined in the Your Custom Parameter form.

Finally, click on the Close button.

The functions knp and kpn will be referred in defining the transition rates for the myosin head in Defining transitions with the cooperative behaviors of the myosin heads.